Rapid Immunofiltration Assay of Francisella Tularensis.
Abstract
A rapid immunofiltration assay for a killed bacterial preparation of Francisella tularensis was developed. Formalin treated cells of Francisella tularensis were incubated with polyclonal antibody to Francisella tularensis and an anti-species antibody conjugated to urease. The reaction mixture was filtered through albumin coated 0.45 micron nitrocellulose membranes following incubation periods which varied from 1 to 60 min. The pH sensing capability of the detector, a light-addressable potentiometric (LAP) sensor, was employed to detect the presence of immobilized urease-conjugated antibodies. The time required for the assay was about 5 minutes exclusive of the incubation period. Limits of detection (LOD) of the assay determined as a function of the incubation time were 1.2 x 10 5 x 10 3 and 3.4 x 103 cells for incubations of 1, 5, and 60 minutes. The speed and ease of the assays suggest that the LAP sensor technology is suitable as a detection system for a mobile field laboratory. Immunofiltration, Filtration, Immunoassay, Antibody, Francisella tularensis, Bacteria, Urease, Nitrocellulose.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1992
- Accession Number
- ADA249991
Entities
People
- H. G. Thompson
- William E. Lee
Organizations
- Defence Research and Development Canada