Protection Against the Acute and Delayed Toxicities of Sulfur Mustard
Abstract
We are investigating DNA repair as a cellular defense against SM toxicity, seeking conditions that will enhance its effectiveness. One repair mechanism, nucleotide excision repair, provides significant protection against toxicity in cultured Chinese hamster ovary cells. Base excision repair by formamidopyrimidine-DNA glycosylase is also effective in vitro, and based on the literature, should be effective in vivo. Human alkylpurine glycosylases are also being cloned and tested for activity. Previous studies have shown that mild hypothermia provides protection against cytotoxicity; we believe this is because hypothermia slows progression through the cell cycle and allows more time for repair to occur before cell division takes place. We have found that hypothermia-induced cell cycle arrest is general in nature, involving both G1 and G2 arrest, as well as a decreased rate of DNA synthesis in S. Hypothermia-induced cell cycle arrest is accompanied by an increase in levels of the protein factors, p2l and p53 and is dependent on the presence of functional p53.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1998
- Accession Number
- ADA351858
Entities
People
- David B. Ludlum
Organizations
- University of Massachusetts