Involvement of Nuclear Receptor Co-Repressors in the Development of Human Breast Cancer.
Abstract
Retinoic acids (RA) inhibit proliferation of many breast cancer cells. Retinoic acid receptors are present in many different breast cancer cells, presumably to mediate such anti proliferation effects of RA. In the absence of RA, the receptor represses transcription through physical interaction with nuclear receptor corepressors SMRT and N-CoR, which form multi-protein complexes containing histone deacetylation activity. In this project, we planned to investigate the expression and regulation of SMRT in breast cancer cells and its role in the cross talk between retinoids and steroids. So far, we have established several breast carcinoma cell lines in the laboratory. We have determined SMRT level in these cells by using Northern and Western blot techniques. We found that most breast cancer cells express SMRT and, interestingly, the cancerous cells contain higher levels of SMRT than the normal breast epithelial cells. These results suggest that SMRT may be involved in regulation of breast carcinoma cell growth and proliferation. Work is currently in progress to understand the role of SMRT in the development of breast cancers and in the cross interaction between retinoids and steroids in this disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA362658
Entities
People
- J. D. Chen
Organizations
- University of Massachusetts