Cataloging Differentially Expressed Sequence Tags from Human Breast Cancer.

Abstract

Using substantial modifications of the differential display polymerase chain reaction technique, we have successfully implemented a moderate-throughput method for isolating and directly sequencing novel cDNA fragments. An initial set of these cDNA fragments correspond to mRNAs that are differentially expressed among five different human breast tumor cell lines. In this report, we summarize the strategy used to create a 'catalog' of approximately 150 of these Differentially Expressed Sequence Tags (DESTs). An outline for work to continue in the second year of this award includes expression analysis of already isolated tags via the creation of a cDNA array and further identification of additional, novel sequence tags from microdissected human breast tumor specimens.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1998
Accession Number
ADA364240

Entities

People

  • Mark A. Watson

Organizations

  • Washington University in St. Louis

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cells
  • Chain Reactions
  • Chemical Reactions
  • Databases
  • Diseases And Disorders
  • Dna Microarrays
  • Gene Expression
  • Identification
  • Mrna
  • Neoplasms
  • Nucleotides
  • Polymerase Chain Reaction
  • Sequence Analysis
  • Sequences
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.
  • Systems Analysis and Design