Growth Suppressors of Breast Cancer Cells
Abstract
A genetic system has been established for the isolation of candidate tumor suppressor genes in MCF7 breast cancer cells. This system includes the IPTG-inducible episomal vector, pEpiLac, which combines the advantages of inducible expression systems and extrachromosomal replication systems. pEpiLac vectors expressed exogenous genes more efficiently than nonepisomal vectors. A cDNA library from normal human breast epithelial cells was constructed in the pEpiLac vector system. The LAP5 cell line, which supports inducible expression of the pEpiLac vector, was derived from human breast cancer MCF7 cells by the introduction of the LAP267 transactivator. Using the episomal vector system, we have conducted a selection for growth inhibitory cDNAs from breast epithelial cells in the LAP5-MCF7 breast cancer cell line. To date, about 12 candidate sequences have been isolated through two rounds of selections. These candidate tumor suppressors are now being characterized structurally and functionally.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1998
- Accession Number
- ADA364727
Entities
People
- Lester F. Lau
Organizations
- University of Illinois at Chicago