Mammary Specific Expression of CRE Recombinase Under the Control of an Endogenous MMTV LTR: A Conditional Knock-Out System.
Abstract
The project to test expression of Cre recombinase directed by the Mtv-l7 locus is in its first year of three years of funding. The hypothesis of the project was to develop a novel and useful breast cancer model using the tissue specific expression of the Mtv-17 locus, which has previously been shown to express only in the mammary gland. We proposed to knocking-in' Cre recombinase at the Mtv-17 env region and this should result in expression of this enzyme only in the mammary gland. Mice that carry the Mtv-l7 Cre fusion vector will be mated to lox P p53 conditional knock-out mice, resulting in loss of functional p53 in the mammary gland only. These animals should only develop mammary tumors and not other tumors. Our knock-in vector will provide a conditional knock-out system and locus for mammary gland specific expression of many different transgenes and should be of widespread utility to the study of both transformation and development of this tissue.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1999
- Accession Number
- ADA366895
Entities
People
- Reb J. Russell Ii
Organizations
- University of Pennsylvania