Identification of Novel Oncogenes in Carcinoma of the Prostate
Abstract
A cell line termed RK3E provides a novel approach to identification and functional analysis of carcinoma-derived transforming oncogenes. Derived by immortalization of primary rat kidney cells, RK3E exhibit multiple properties of epithelia and specifically detect the transforming activity a small subset of all oncogenes, including c-MYC, RAS, (beta)-catenin, and the zinc finger proteins GLI and GKLF. Strikingly, the conventional host for oncogene isolation, NIH3T3 cells, detects the activity of just one of these carcinoma-relevant oncogenes, the G protein RAS. GKLF was recently identified as a novel RK3E transforming oncogene and was found to be expressed at elevated levels in dysplastic epithelium in vivo. These results suggest that carcinoma types for which oncogenes are poorly characterized can be rapidly assessed for novel transforming oncogenes, and that such genes are likely to be relevant to tumor progression in vivo. For analysis of oncogenes expressed in prostate cancer, we have purified polyA+ mRNA and begun preparation of cDNA libraries.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA380401
Entities
People
- John M. Ruppert
Organizations
- University of Alabama