Inhibition of Invasiveness and Motility of Human Breast Cancer Cells by Sphingosine-1-Phosphate

Abstract

Sphingosine-1-phosphate (SPP), a bioactive sphingolipid metabolite, inhibits chemoinvasiveness of the aggressive, estrogen independent MDA-MB-23 1 human breast cancer cell line. Similar to many other cell types, SPP stimulated proliferation of MDA- MB-231 cells, albeit to a lesser extent. Treatment of MDA-MB-231 cells with SPP had no significant effect on their adhesiveness to Matrigel, and only high concentrations of SPP partially inhibited matrix metalloproteinase 2 activation induced by Con A. However, SPP at a concentration that strongly inhibited invasiveness, also markedly reduced chemotactic motility. To investigate the molecular mechanisms by which SPP interferes with cell motility, we examined tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin, which are important for organization of focal adhesions and cell motility.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1999
Accession Number
ADA382431

Entities

People

  • Fang Wang

Organizations

  • Georgetown University

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Health Services
  • Lymphocytes
  • Organic Chemistry
  • Peptide Growth Factors
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Cellular and Molecular Pathways of Apoptosis.
  • Molecular Biology and Genetics