Studies on Liver DNA in Tritiated Kangaroo Rats Living at Sedan Crater
Abstract
Kangaroo rats (Dipodomys merriami) living near the crater of an old nuclear detonation were found to be uniformly tritiated. The lifetime chronic dose to adult animals was estimated to be 10 rad from internal 3H and 10 rad from persistent external Beta- and Gamma emitting radionuclides. Liver DNA was isolated from these animals and from control animals trapped in areas not enriched in radionuclides. DNA from the crater animals contained 106 6 X dpm/g hydrogen. In comparison with control DNA, the crater DNA exhibited the following: more protein and histone, higher molar absorbance at 260 micronmeter lower thermal transition magnitude with a small secondary transition, lower mole fraction of A + T nucleotides, increased template activity for 2 RNA polymerases and 1 DNA polymerase, and relative increase in ATP-gamma-32P incorporation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 10, 1968
- Accession Number
- ADA382770
Entities
People
- F. T. Hatch
- G. G. Greenway
- J. A. Mazarimas
- J. J. Koranda
- J. L. Moore
Organizations
- University of California