Human Adrenal Androgens: Regulation of Biosynthesis and Role in Estrogen-Responsive Breast Cancer in a Mouse Model

Abstract

These experiments investigate a mouse model for the biosynthesis of the human adrenal androgens (dehydroepiandrosterone, DHEA, and its sulfate, DHEAS) and the role of these steroids in human breast cancer growth. An androgen-dependent human breast cancer model was established in immunodeficient (scid) mice. Zona reticularis cells in the human adrenal cortex are responsible for adrenal androgen biosynthesis because of the suppressed expression of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) in these cells. A protein present in the non-DHEA-secreting zones of the cortex and absent from the zona reticularis which binds to a regulatory region of the type II 3 beta-HSD gene was partially purified. Human adrenocortical cells were transplanted into scid mice and were shown to replace the animals' own adrenal function.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1999
Accession Number
ADA383881

Entities

People

  • Peter Hornsby

Organizations

  • Baylor College of Medicine

Tags

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Endocrine Glands
  • Health Services
  • Medical Personnel
  • Polymeric Films

Fields of Study

  • Biology

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  • Immunology
  • Molecular and Cellular Biochemistry
  • Prostate Cancer Biology.

Technology Areas

  • Biotechnology