Expression Profiling of Tyrosine Kinase Genes
Abstract
There is strong evidence that the expression of genes involved in signal transduction such as protein kinases is altered in tumor cells and that the aberrant expression of one or several of these genes parallels the progression of tumors to a more malignant phenotype. We developed a DNA micro-array based screening system to monitor the level of expression of tyrosine kinase (tk) genes and to derive quantitative information to support prognostication and therapeutic decisions. In the first year of this effort, we finished the development and testing of hardware necessary to prepare the DNA micro-arrays, performed RNA extraction, cDNA preparation and labelling reactions, and defined a working protocol for array hybridization and quantitative analysis. Our prototype design simultaneously measures the expression of 58 different tk genes. Additional targets for investigation will be defined by the ongoing molecular cloning and sequencing of gene transcripts found in breast cancer cell lines and clinical specimens. Using a set of phenotypically well characterized breast cancer cell lines, the system has proven to be able to deliver reproducible data regarding changes in tk gene expression during cell transformation and progression towards a more malignant phenotype.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2000
- Accession Number
- ADA385743
Entities
People
- Heinz U. Weier
Organizations
- University of California, Berkeley