Involvement of the Tyrosine Phosphatase SHP-1 in the Development of Breast Cancer
Abstract
The purpose of this research was to test the working hypothesis that SHP-1 is essential for controlling growth and differentiation of mammary epithelial cells and that dysregulation of SHP-1 contributes to the development of breast cancer. Scope: To biochemically and functionally characterize SHP-1 in human breast cancer cell lines and to define the biological function of SHP-1 in normal epithelial cells. Major Findings: SHP-1 associates with the EGFR in an EGF stimulation-dependent manner. To further characterize the function of SHP- 1, we have generated retroviruses encoding wild type and mutant forms of SHP- 1 together with GFP. Infection of cell lines with these viruses will enable us to target a high percentage of the cell population and to identify infected cells. We have also generated a transgenic mouse encoding SHP-1 under the control of its hematopoietic promoter. Crossing this mouse in the motheaten background should allow us to study SHP- 1-deficient epithelial cells in an otherwise "normal" mouse. Significance: From our data, we are starting to understand SHP-1's role in epithelial cells. Moreover by using the reagents we generated, we expect to deepen our knowledge of SHP-1's role in epithelial cells and to learn how a dysregulated SHP-1 is potentially involved in the onset/progression of breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADA385886
Entities
People
- Ulrike Lorenz
Organizations
- University of Virginia