Response of Breast Cancer Cells to Hormonal Therapy; Quantitative in Vivo NMR Studies

Abstract

Our research is directed at determining the specific metabolic pathways and the individual reactions affected by estrogen and tamoxifen in both estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) breast cancer cells through the use of novel NMR methodology and quantitative analysis. In this report period, we have quantified the effect of estrogen on metabolic fluxes in ER+ cells. This was accomplished using a comprehensive metabolic model and NMR data from cell extracts. The model was further refined to allow for the incorporation of isotopomer, or multiply-labeled, metabolite data. Microcarrier culture, flask, and roller bottle studies were continued in order to expand the methodology used to quantify breast cancer cell metabolism under varied treatment conditions. In addition, investigations were performed in flasks to characterize the dose response of cells to the metabolic inhibitor cerulenin.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1999
Accession Number
ADA386520

Entities

People

  • Douglas S Clark

Organizations

  • University of California, Berkeley

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Amino Acids
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Cytological Techniques
  • Estrogens
  • Fatty Acids
  • Liquid Chromatography
  • Materials
  • Measurement
  • Metabolic Pathways
  • Metabolism
  • Metabolites
  • Neoplasms
  • Tissue Extracts

Readers

  • Breast cancer cell signaling and growth regulation.
  • Computational Modeling and Simulation
  • Microbial Pathology