Development of Integrase-Like Catalytic Antibodies for Breast Cancer
Abstract
This project aims to develop catalytic antibodies that will allow efficient DNA transfer by promoting DNA site-specific recombination. Such antibodies may provide a very powerful means to manipulate DNA transfer at defined sequences with obvious implications for gene therapy of breast cancer. In order to engineer enzymes that catalyze the recombination, we immunized mice with a synthetic DNA Holliday structure and screened over 5,000 hybridomas. Since last report, we have screened additional 200 hybridomas and had two primary supernatants contained substances that bound to the structure specific to its cruciform junction. Again we could not isolate the clones that possess the properties after expansion and purification. We also screened over 500 clones from Fab expression library and no desired clones were obtained. This is a very technical and labor challenging project. We feel that our resources are limited to successfully complete the project. Successful completion of this project will provide a new technology of DNA transfer for both biomedical research and therapeutic purposes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADA388930
Entities
People
- E. Aubrey Thompson
Organizations
- University of Texas Medical Branch