Altering Ets Activity to Reverse Transformation of Breast Cancer Cells
Abstract
The Ets family of transcription factors are downstream targets of several activated signal transduction steps identified in breast tumors. Interfering with Ets-dependent transcription by expression of the Ets2 DNA binding domain (Ets2DBD) reverses Ras-mediated NIH3T3 cell transformation. We hypothesized that altering Ets activity could also effectively reverse the transformed phenotype of breast cancer cells. We analyzed the effects of stably expressing different Ets2 constructs in Ras-transformed NIH3T3 (DT-3T3) cells and in a human breast cancer cell line, MDA-MB-435. Expression of the dominant inhibitory Ets2DBD caused loss of anchorage independent growth in both cell types.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2001
- Accession Number
- ADA391338
Entities
People
- Gabriele E. Foos
Organizations
- Sanford Burnham Prebys Medical Discovery Institute