Involvement of Reactive Oxygen Species in Breast Cancer Cells Development, Maintenance and Death
Abstract
Oxygen free radicals (OFR) have different effects on cellular processes. They were implicated in cell damage, apoptosis, and carcinogenesis. The hypothesis in this proposal is that the effect of OFR on cells is concentration dependent. At high concentration OFR cause damage, at lower concentration they cause apoptosis through the TNF-a signal transduction pathway, at even lower concentration they fail to activate the apoptotic signal, leading to the development of cancer. To test this hypothesis we proposed to measure the level of OFR in normal and cancerous breast cell lines and primary human breast tissue, and to correlate the OFR concentration with apoptosis and carcinogenesis. We have established and furnished a state of the art a tissue culture and breast cancer research laboratory. In this lab we established we established breast cell lines - normal and cancer, and developed tissue culture capabilities for primary breast cells. In collaboration with Dr Zeev Rosenzweig at the University of New Orleans we developed sensitive, precise, and reproducible methods to measure oxygen and oxygen free radicals in single cells. Experiments designed to measure the level of OFR in breast cells are currently underway.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2001
- Accession Number
- ADA396417
Entities
People
- David Jansen
- Kim O'connor
- Nitsa Rosenzweig
Organizations
- Xavier University of Louisiana