Oxidases as Breast Cancer Oncogens
Abstract
This proposal examines the novel concept that H2O2 generating oxidase-mediated reactive oxygen species in breast epithelium contribute to the development of breast cancer. Constructs to express xanthineoxidase under the direction of mouse mammary tumor virus (NNTV) promoter were generated. Transfections performed to generate cell lines stable expressing the enzyme were unsuccessful hence urate oxidase was used as an alternative . The choice for urate oxidase is based on the fact that we have used this enzyme in previous studies and showed that cells expressing this enzyme reveal the characteristic crystals of this enzyme in peroxisomes. Tranagenic mice expressing UOX under the transcriptional control of MMTV promoter were generated. We have injected the construct in fertilized ova and identified five founder mice initially which failed to transmit the transgene. Additional microinjections generated 9 founders. Five of these were found to express the transgene. Southern, Northern and Western analyses showed the presence of the transgene in the mammary, and testicular tissues. We will now initiate studies to examine the role of reactive oxygen species in causing cell death, cell proliferation and neoplastic transformation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2001
- Accession Number
- ADA396460
Entities
People
- Anjana Yeldandi
Organizations
- Northwestern University