Protein-Based Three-Dimensional Memories
Abstract
Significant progress has been made in the remaining tasks, specifically materials development, protein optimization, computer interface development, and prototype development. The primary goal of the materials effort was to develop a polymer matrix for encapsulation of the protein, characterized by optical clarity, long-term stability, protein compatibility, and resistance to gel dehydration and contraction. Handled via subcontract to Wayne Wang of Carleton University in Ottawa, Canada, a poly(acrylamide) based hydrogel has been developed that fits the majority of these characteristics. Light scattering was achieved primarily by the addition of refractive index-matching agents, with sucrose achieving the best reduction. A newly developed high-density acrylamide matrix demonstrates the largest reduction of light scattering, by roughly one order of magnitude over previous gels. Optimization of the protein response was approached primarily through site-directed mutagenesis (SDM), with the goal of increasing the efficiency with which the branched photocycle can be assessed; two avenues were explored to this end, including enhancement of the 0-state yield and the quantum yield of the 0-> P transition. The former avenue increases the amount of P-state formed through simple mass transfer-a higher yield of 0 will result in more P formation, despite the 0-> P quantum efficiency. The latter approach seeks to directly increase the 0-> P quantum efficiency.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2002
- Accession Number
- ADA399541
Entities
Organizations
- Syracuse University