High Temperature Superconductor RF Probes for Breast Cancer Research
Abstract
Nuclear magnetic resonance imaging and spectroscopy techniques have been used to study the breast cancer cells and tumor grown on animal. The goal of this study is to gain knowledge, which could be useful to improve the specificity of diagnosis and staging of breast cancer in clinic. Intrinsically, NMR techniques have weak signals, which limit the ultimate spatial resolution in image and sensitivity in spectrum. A high temperature superconductor RF probe has been constructed, which has a Q value 650, to improve NMR sensitivity. An improved cell perfusion system was made to continuously run spectroscopy study longer than 8 days. Many phosphorus metabolites of MCF7 breast cancer cells have been identified. There is a subtle difference between MCF7/WT and MCF7/ADR cells. Glycerophosphocholine is lower than phosphocholine for the MCF7/ADR cells. 2 Micro-M Doxorubicin dramatically affected MCF7/WT cells but not MCF7/ADR cells. T1 relaxation times of the phosphorus metabolites are measured. The NMR spectrum shows significant lower PC and ATP and higher Pi signals in tumor than the muscle. High-resolution imaging technique with injection of contrast agent shows a significant non-uniform image enhancement throughout the tumor. Small blood vessels can be identified at peripherals but not at the core of tumor.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2001
- Accession Number
- ADA404908
Entities
People
- Paul C Wang
Organizations
- Howard University