Miniaturized DNA Biosensor for Decentralized Breast-Cancer Screening

Abstract

The use of DNA testing as an important component of breast cancer diagnosis has been increasing rapidly over the past decade. The goal of this project is to develop and characterize an electrochemical biosensing microsystem for the rapid point-of-care genetic screening of breast-cancer. During the second year of this project we introduced innovative electrochemical routes for improving the reliability of devices for genetic screening of breast-cancer. In particular, we have successfully combined the unique amplification features of DNA accumulation or metal nanoparticle networks, with an effective magnetic isolation of the duplex, and a powerful label-free electrical detection for achieving the task of sensitive and selective breast-cancer diagnostics. Additional developmental work, particularly further improvements in the sensitivity and selectivity (through the use of dendritic and PNA probes) along with transforming to a miniaturized flow system, is in progress towards the realization of wide-scale decentralized screening for breast cancer.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2002
Accession Number
ADA406787

Entities

People

  • Joseph Wang

Organizations

  • New Mexico State University

Tags

Communities of Interest

  • Biomedical
  • Sensors

DTIC Thesaurus Topics

  • Biochemistry
  • Biosensors
  • Buffers (Chemistry)
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Detectors
  • Dna Biosensors
  • Electrochemical Cells
  • Genetic Testing
  • Glucose Monitors
  • Materials
  • Metallic Nanoparticles
  • Metals
  • Nanoparticles
  • Nucleic Acids
  • Point-Of-Care Diagnostic Testing

Readers

  • Molecular Genetics
  • Nanoscale Plasmonic Nanotechnology
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology