DNA Base Excision Repair (BER) and Cancer Gene Therapy: Use of the Human N-Methylpurine DNA Glycosylase (MPG) to Sensitive Breast Cancer Cells to Low Dose Chemotherapy
Abstract
To ensure that the genome is not compromised, elaborate mechanisms of DNA repair are essential to the cell. The DNA Base Excision Repair (BER) pathway is responsible for the repair of alkylation and oxidative DNA damage. The short patch BER pathway begins with the simple glycosylase N- methylpurine DNA glycosylase (MPG), which is removes damaged bases such as N3-methyladenine, hypoxanthine, and 1,N6-ethenoadenine from the DNA. The resulting AP site is further processed by the other enzymes of the BER pathway to complete repair of the damaged DNA. Using mammalian expression vectors for stable and adenovirally-mediated transient overexpression, MPG has been overexpressed in two breast cancer cell lines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA407383
Entities
People
- Mark R. Kelley
- Mikael L. Rinne
Organizations
- Indiana University