Use of DNA Microarrays to Identify Diagnostic Signature Transcriptional Profiles for Host Responses to Infectious Agents
Abstract
Most of the likely agents of bio-terrorism have profound effects on the host and, in particular, on the immune and inflammatory responses. We have proposed a broad-based approach to identify the unique "signatures" of infectious agents using host DNA microarrays. Because of the known diverse patterns of host cell interactions with these organisms, examination of the host transcriptional response has enormous potential to allow rapid diagnosis of infectious diseases in general and agents of bio-terrorism in particular. Key signature genes will serve as the basis for rapid diagnostic approaches that could be accessed when an outbreak is suspected. The agents under initial investigation are Bacillus anthracis, Burkholderia Mallei, Francisella tularensis, multi- drug resistant Mycobacterium tuberculosis and Yersinia pestis. We have developed appropriate human and mouse models to explore the interactions of host cells with the pathogen.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2003
- Accession Number
- ADA419551
Entities
People
- Jerrold J. Ellner