Interrogating Androgen Receptor Mediated Gene Expression and Tumor Progression by Molecular Imaging

Abstract

The main goal of the project is to develop a transcription-based optical imaging system for studying androgen receptor (AR) function during prostate cancer progression in animal models. We have now developed a system termed two-step transcriptional activation (TSTA) and used it to show that AR is functional in the androgen independent (AI) phase of the cancer. Mechanistic analyses showed that AR was localized to the nucleus, bound its enhancers and assembled transcription complexes in AI cancers. We have also modified the TSTA system to detect elevated levels of mitogen activated protein kinase (MAPK) in cell culture and in AI animal models of prostate cancer. We are employing the MAPK optical imaging system to study the transition of prostate cancer from androgen dependence to AI. preliminary data suggest that AI cancer contains markedly elevated levels of MAPK compared with AD cancer.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2003
Accession Number
ADA419790

Entities

People

  • Michael F. Carey

Organizations

  • University of California, Los Angeles

Tags

DTIC Thesaurus Topics

  • Androgen Receptors
  • Biological Sciences
  • Breast Cancer
  • Carcinoma
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Gene Expression
  • Gene Therapy
  • Genetics
  • Neoplasms
  • Prostate Cancer
  • Proteins
  • Tissue Extracts
  • Tissues
  • Tumor Cell Line

Fields of Study

  • Biology
  • Physics

Readers

  • Breast cancer cell signaling and growth regulation.
  • Medical Imaging.
  • Prostate Cancer Biology.