Polymerase Chain Reaction for the Rapid Detection of Bulkholderia pseudomallei

Abstract

Melioidosis is a potentially lethal infection endemic in Northern Australia and Southeast Asia. The causative bacterium, Burkhoideria pseudomallei, is resistant to many of the commonly used antibacterial agents. Therefore, successful treatment of the disease is dependent on rapid and accurate diagnosis. The current diagnostic techniques are time consuming and lacking in specificity. We have developed a PCR procedure using the R.A.P.I.D(trademark) and real time monitoring of product formation. The reaction is significantly faster than conventional PCR and allows the simultaneous evaluation of specificity using melting curve analysis.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2003
Accession Number
ADA423659

Entities

People

  • Matthia Dorsch
  • Susan Shahin

Organizations

  • Defence Science and Technology Group

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Australia
  • Bacteria
  • Cells
  • Chain Reactions
  • Chemical Biological Radiological And Nuclear Defense
  • Chemical Reactions
  • Diseases And Disorders
  • Fluorescence
  • Fluorescent Dyes
  • Identification
  • Infection
  • Polymerase Chain Reaction
  • Thermal Cyclers
  • Universities
  • Warfare
  • Wound Infections

Readers

  • Microbial Pathology
  • Oncology and Biomarker-Based Cancer Detection.