The Role of Akt in Androgen-Independent Progression of Human Prostate Cancer
Abstract
To define the causative role of the active Akt in androgen-independent progression of prostate cancer, an inducible Akt (iAKT) is used in this proposal. In this first-year period, androgen-dependent human prostate cancer LNCaP cells Were transfected with vectors carrying iAKT system or the control vectors (empty and kinase-dead mutant iAKTkm) to establish stable sublines. We established multiple LNCaP sublines expressing the iAKT system construct, as well as the control vectors. We demonstrated that the established LNCaP.iAKT cells showed a promising ability that the iAKT can be activated after the cells were treated by the dimerization inducer AP22783, and the activated iAKT (F(pk)3-delta phAKT) is functionally capable to perform its physiological task, phosphorylating the downstream target OSK-3. In the next year or step, we will used those LNCaP subline cells to determine whether CID-mediated activation of iAKT promotes survival/proliferation of prostate cancer LNCaP cells after androgen withdrawal.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2004
- Accession Number
- ADA423768
Entities
People
- Benyi Li
Organizations
- University of Kansas Medical Center