The BioFlip Microcytometer
Abstract
This four-year program successfully demonstrated a point-of-care (POC) integrated scattering and fluorescence flow cytometer capable of counting and classifying white blood cells from a whole-blood sample input. The instrument development and overall system testing tasks on this project were performed by Honeywell and the disposable cartridge development was led by Micronics. The revolutionary miniaturization of a cytometer was enabled by the core technology strengths of Honeywell and Micronics. As part of this project, the Honeywell-led team has demonstrated three-part differentiation of white blood cells using a 6 in. x 6 in. x 4 in., 3-lb light-scattering-based cytometer. The cytometer is composed of a handheld instrument and credit-card-sized disposable analysis cartridges. The system has the following features: automated sample preparation on the cartridge, red-VCSEL-array-based electronic selfalignment, highly miniaturized multi-channel pumping system, and custom-developed electronics and graphic user interface. In addition, the team has demonstrated a highly miniaturized integrated two-color light-fluorescence based cytometer. Repeatable counting and classification of two-color bead mixtures was demonstrated in this cytometer. This handheld, easy to use instrument can be modified for the detection of biowarfare agents in environmental samples.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 10, 2004
- Accession Number
- ADA430744
Entities
People
- Aravind Padmanabhan
Organizations
- Honeywell International, Inc.