Activation of Retinoid X Receptors by Phytanic Acid and Docohexaenoic Acid: Role in the Prevention and Therapy of Prostate Cancer
Abstract
In this study we investigated the effects of these two dietary RXR ligands (agonists) on the cell growth and retinoid metabolism of cultured human prostate cancer cell lines. Retinoic acid (RA) treatment for 72 hours and 120 hours did not have a significant effect on the growth of PC-3 cells. However, RA did suppress the proliferation of LNCaP cells, especially after 120 hours. The specific RXR agonist BMS 188649 inhibited cell proliferation in both cell lines. Meanwhile, both phytanic acid and DHA inhibited the growth of Pc-3 and LNCaP cells. Phytanic acid and retinoic acid synergistically inhibited the growth of both of these prostate cancer cell lines. In addition, Western blot analysis showed that both phyanic acid and DHA decreased cyclin Dl expression in PC-3 cells. We also examined retinol (vitamin A) metabolism in PC-3 cells treated with phytanic acid and DHA by HPLC. Both phytanic acid and DHA altered the metabolism of retinol. These results suggest that both phytantic acid and DHA, natural dietary RXR ligands, may be useful agents for future dietary preventive and therapeutic approaches to human prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2005
- Accession Number
- ADA431257
Entities
People
- Xiao-han Tang
Organizations
- Cornell University