A Novel Spectroscopic Methodology for the Investigation of Individual Bacillus Spores

Abstract

A methodology has been developed for the investigation of bacterial spores. Specifically, this method has been used to probe the spore coat composition of two different Bacillus stearothermophilus variants. This unique detection scheme is based on the NIR Surface-Enhanced-Raman-Scattering (SERS) from single optically trapped, bacterial spores. The light from a 787-nm laser diode was used to trap/manipulate as well as simultaneously excite the SERS of an individual bacterial spore. Comparison of normal Raman and SERS spectra reveal not only an enhancement of the normal Raman spectral features but also the appearance of spectral features absent in the normal Raman spectrum.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2005
Accession Number
ADA449687

Entities

People

  • James B. Gillespie
  • Paul Pellegrino
  • Troy A. Alexander

Organizations

  • United States Army Research Laboratory

Tags

DTIC Thesaurus Topics

  • Charge Coupled Devices
  • Detection
  • Electric Fields
  • Energy Bands
  • Excitation
  • Laser Beams
  • Laser Diodes
  • Lasers
  • Optical Lattices
  • Raman Scattering
  • Raman Spectra
  • Raman Spectroscopy
  • Resonance
  • Scattering
  • Spectra
  • Spores
  • Surface Plasmon Resonance

Readers

  • Image Processing and Computer Vision.
  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Quantum spin resonance or Electron Paramagnetic Resonance spectroscopy.

Technology Areas

  • Directed Energy