Acetylcholinesterase-Based Electrochemical Multiphase Microreactor for Detection of Organophosphorous Compounds (Preprint)

Abstract

A dual microchannel device with a gas-liquid interface was developed for use as an amperometric biosensor for the detection of organophosphorus compounds based on acetylcholinesterase inhibition. Electric eel acetylcholinesterase was immobilized on the liquid microchannel by creating a cross-linked gel with glutaraldehyde. The system was tested with malathion, an organophosphorus pesticide. The detection limit of the sensor in the parts-per-trillion range and the detection is rapid, sensitive, and selective to only phosphonates. Incorporation of existing acetylcholinesterase biochemistry into a micro-scale sensor also allows the device to be easily portable.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2007
Accession Number
ADA471414

Entities

People

  • Chelsea N. Monty
  • Iiwhan Oh
  • Richard I. Masel

Organizations

  • University of Illinois Urbana–Champaign

Tags

Communities of Interest

  • Sensors

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Air Force
  • Air Force Research Laboratories
  • Albumins
  • Biochemistry
  • Chemical Engineering
  • Chemical Synthesis
  • Chemical Warfare Agents
  • Chemistry
  • Detection
  • Detectors
  • Engineering
  • Enzymes
  • Fuel Cells
  • Inhibition
  • Organophosphorus Compounds
  • Pesticides

Fields of Study

  • Agricultural and Food sciences
  • Chemistry

Readers

  • Analytical Chemistry
  • Nanoscale Plasmonic Nanotechnology
  • Neurotoxicology

Technology Areas

  • Biotechnology