UF Biomotor/Biosensor Nanotechnologies
Abstract
This report contains a summary of activities on the project "UF Biomotor/Biosensor Nanotechnologies". Nanoscale actuators for use as molecular shuttles in biosensing devices were developed based on actin filament end-tracking motors. Novel strategies were developed exploiting these motors and modified substrata to propel and guide motor-coated micro- and nanoparticles using substratum-bound actin filaments with their elongating plus-ends bound to the particle surface. Key accomplishments of this project include (1) optimization of conditions for particle propulsion in cell extracts, (2) development of single-filament actuators, (3) guidance of single-filament elongation on patterned and microfabricated substrata, (4) development and validation of a mathematical model that predict particle propulsion velocity as a function of controllable parameters, (5) novel time-of-flight mass spectrometry methods to image surfaces, and (6) direct real-time methods to observe protein-protein interactions involved in filament end-tracking in vivo.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 25, 2007
- Accession Number
- ADA472921
Entities
People
- Brian Holiday
- Colin Sturm
- Daniel L. Purich
- Denis Wirtz
- Gary Mcguire
- Kimberly Interliggi
- Richard B. Dickinson
- Suzanne Hens
- William Cooke
- William Zeile
Organizations
- University of Florida