Markers of Hypoxia/Reoxygenation in the Development of Metastatic Breast Cancer

Abstract

A novel in vitro cell perfusion system was designed and constructed. This system can maintain cultured breast cancer cells under conditions simulating the hypoxia/reoxygenation cycles observed in vivo. Preliminary studies suggest that breast cancer cells grown under hypoxia undergo oxidative stress as the main cellular antioxidant glutathione is depleted under these conditions. This suggests that hypoxia and reoxygenation may trigger development of the metastatic phenotype and that glutathione may be a marker for the early stages of progression.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2007
Accession Number
ADA473777

Entities

People

  • Michael P. Gamcsik

Organizations

  • Duke University

Tags

DTIC Thesaurus Topics

  • Biomedical Engineering
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Culture Techniques
  • Cultured Cells
  • Department Of Defense
  • Electrodes
  • Flow Rate
  • Membranes
  • Neoplasms
  • Oxidative Stress
  • Perfusion
  • Tissue Culture
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Computational Linguistics
  • Oncology (Cancer Research).
  • Prostate Cancer Biology.