Stromal-Epithelial Interactions and Tamoxifen-Sensitivity: A Bench-to-Beside Model of Chemoprevention

Abstract

Funding of this multidisciplinary training program supports efforts to 1) use Random Periareolar Fine Needle Aspiration (RPFNA) to test for correlations between a) hyper-methylation of estrogen responsive genes b) estrogen responsive protein expression by immunohistochemistry (IHC) and a cytological response to tamoxifen chemoprevention and then 2) test whether resistance to tamoxifen correlates with lack of co-activator recruitment to an estrogen response element (ERE) in state-of-the-art preclinical models. We have established and validated methylation specific PCR primers for the detection of two isoforms of the Progesterone Receptor in Random Periareolar Fine Needle Aspiration (RPFNA) samples. Likewise we have developed protocols to evaluate the steroid hormone concentration in RPFNA samples. Preliminary investigation of steroid concentration indicates that there may be a positive correlation between breast tissue estradiol concentration and cytological atypia.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2007
Accession Number
ADA474719

Entities

People

  • Craig Rowell

Organizations

  • Duke University

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Breast Cancer
  • Carcinoma
  • Cell Line
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Drug Therapy
  • Epithelial Cells
  • Gas Chromatography
  • Hormones
  • Mammary Glands
  • Mass Spectrometry
  • Medical Personnel
  • Neoplasms
  • Spectra

Readers

  • Breast cancer cell signaling and growth regulation.
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology