Uncovering the Mechanism of ICI-Mediated Estrogen Receptor-Alpha Degradation
Abstract
A cytoplasmic ER variant (lacking the NLS), expressed in MCF-7 cells, is resistant to ICI-mediated degradation up until 12hr. At 24hr, this protein may start to degrade, so temporal effects of ICI on cER degradation will have to be further explored. Interestingly, this cytoplasmic ER variant may actually be stabilized by ICI after 6hr of treatment. A rhodopsin-fused full-length ER-alpha (containing its NLS) that is sequestered at the plasma membrane might be degraded by increasing concentrations of ICI in MCF-7 cells. Furthermore, this RhER construct may be post-translationally modified by ICI but not by estradiol.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2007
- Accession Number
- ADA477035
Entities
People
- Angelo Casa
Organizations
- Baylor College of Medicine