Base-Dependent Competitive Adsorption of Single-Stranded DNA on Gold

Abstract

Single-stranded DNA probes (ssDNA) immobilized on gold surfaces are a common element in many biotechnology and nanotechnology applications. Although recent studies have shown that different DNA bases and homo-oligonucleotides interact differently with Au surfaces, competitive interactions among the bases, which will occur in most practical applications, have not been systematically addressed. Here, we examine room-temperature adsorption of homo-oligonucleotides onto polycrystalline Au films, including competitive adsorption between all possible pairs of such unmodified oligomers. Homo-oligonucleotides serve as a model system that allows us to systematically study the base- and length dependence of ssDNA-Au interactions, while maintaining most properties of practical ssDNA probes. We characterize the adsorption ex-situ using Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS), two methods that together provide unambiguous spectral signatures of the different bases and allow absolute surface densities to be determined.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2003
Accession Number
ADA481143

Entities

People

  • Dmitri Y. Petrovykh
  • Hiromi Kimura-suda
  • Llyod J. Whitman
  • Michael J. Tarlov

Organizations

  • National Institute of Standards and Technology

Tags

DTIC Thesaurus Topics

  • Adsorption
  • Aqueous Solutions
  • Desorption
  • Electronic Mail
  • Information Operations
  • Maryland
  • Materials
  • Military Research
  • Mixtures
  • Nanotechnology
  • Oligomers
  • Sorption
  • Spectra
  • Spectroscopy
  • Standards
  • Universities
  • X Ray Photoelectron Spectroscopy

Readers

  • Electrochemical Engineering/ Fuel Cell Technologies
  • Materials Science and Engineering.
  • Thin Film Deposition Science.

Technology Areas

  • Biotechnology
  • Microelectronics