Replicating Viral Particles and other Shape-controlled, Functional Particles for Targeted Delivery Applications Using Nano-molding Techniques
Abstract
The main focus of the work was to explore engineered PRINT particles to elicit an immunological response. The primary objective was to test the idea that engineered PRINT particles could facilitate different T and B cell immune responses. A secondary objective was to use PRINT to make particles from biological and man-made master templates to afford particles of controlled shape down to <1 nm resolution and functionality. Molecular templating agents and viral-envelope proteins were investigated to augment the shape-specificity of our molded nanoparticles. Several strategies for conjugation a wide variety of ligands to the surface of the PRINT particles, including strategies for the binding of nitrophenyl, pneumococcal polysaccharide-C and streptavidin-biotin conjugates were developed. A major advantage of PRINT delivery is the ability to target the cargo to professional antigen presenting cells. Specific and effective targeting to dendritic cells was observed. Also explored was the particle uptake and release of cargo in vitro. PRINT particles have been fabricated that are comprised of disulfide cross-linkers that are sensitive to the reducing environment which allows them to dissolve in cellular vesicles. The results and progress over the period of the grant July, 2006 to July, 2007 are described.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 23, 2007
- Accession Number
- ADA483073
Entities
People
- Jeffrey Frelinger
- Joseph Desimone
- Jude Samulski
- Sergio Sheiko
Organizations
- University of North Carolina at Chapel Hill