An in vitro Corneal Model with a Laser Damage Threshold at 2 Micrometers That is Similar to That in the Rabbit

Abstract

Corneal organotypic cultures were generated as per existing methods, which included growth on polycarbonate inserts and air-lifting for one week. The corneal simulant cultures were exposed, with real-time IR imaging, to the 2-micron wavelength output of a thulium fiber laser with 4 mm beam diameter for 0.25 seconds in a thermally controlled environment and then assayed for damage. The in vitro threshold (ED50 value of 12.5 W/sq cm) and peak temperature (77.5 deg C) at threshold irradiance are compared with rabbit corneal data in the literature.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2007
Accession Number
ADA500272

Entities

People

  • Kurt J. Schuster
  • Larry E. Estlack
  • Michael L. Denton
  • Michael S. Foltz
  • Robert J. Thomas

Tags

Communities of Interest

  • Advanced Electronics
  • Energy and Power Technologies
  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Air Force
  • Air Force Research Laboratories
  • Calibration
  • Cells
  • Confocal Microscopy
  • Culture Techniques
  • Damage
  • Data Analysis
  • Data Sets
  • Diameters
  • Laser Damage
  • Laser Safety
  • Lasers
  • Light Sources
  • Microscopes
  • Optical Fiber Lasers
  • Optics

Readers

  • Pulsed Power and Plasma Physics.
  • Toxicology/Environmental Toxicology

Technology Areas

  • Directed Energy