Contrast Agents for Micro-Computed Tomography of Microdamage in Bone

Abstract

Novel methods have been developed for detecting damaged bone tissue using micro-computed tomography (micro-CT) and contrast agents with higher x-ray attenuation than bone. The ability to non-destructively and three-dimensionally detect the presence, spatial location and accumulation of fatigue microdamage in cortical using micro-CT was demonstrated using a precipitated barium sulfate (BaSO4) stain. Gold nanoparticles (Au NPs) were functionalized with bisphosphonate (alendronate) and carboxylate (L-glutamic acid) ligands, and exhibited stability in physiological solutions, specificity for damaged tissue and a high binding affinity for bone mineral. Synchrotron x-ray tomography was able to detect alendronate and glutamic acid functionalized Au NPs labeling controlled surface damage on cortical bone specimens.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2009
Accession Number
ADA502740

Entities

People

  • Ryan K. Roeder

Organizations

  • University of Notre Dame

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Attenuation
  • Barium Compounds
  • Chemistry
  • Detection
  • Electron Microscopy
  • Glutamic Acid
  • Materials
  • Materials Science
  • Metallic Nanoparticles
  • Nanoparticles
  • Particles
  • Phosphonic Acids
  • Radiation
  • Synchrotron Radiation
  • Tomography
  • X Rays
  • X-Ray Computed Tomography

Readers

  • Medical Imaging.
  • Molecular and Cellular Biochemistry
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech