Role of Fetuin-A in Breast Tumor Cell Growth
Abstract
In this report, we have described the experiments pertaining to task#1 of our original proposal (To elucidate the significance of fetuin-A in breast carcinogenesis). The central hypothesis of this grant is that fetuin-A is a major serum derived growth factor for breast carcinoma cells and creates a favorable environment for the metastatic spread of tumor cells to the lungs. We have successfully placed Fet+/+/PymT+; Fet+/-/PymT+; and Fet-/-/PymT+ female C57 black mice in experimental protocols. Our data show that lack of fetuin-A protects female fetuin-A null PymT+ mice from breast cancer. However, if fetuin-A null mice develop breast cancer, it is mainly squamous carcinoma, a rare breast cancer. We have also made significant progress in task # 2 where we now show for the first time that cell attachment is mediated only by fetuin-A and not its major contaminant which is alpha-2-macroglobulin. We also show both fetuin-A and alpha-2-macroglobulin can activate PI3 kinase/Akt.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2009
- Accession Number
- ADA513186
Entities
People
- Josiah Ochieng
Organizations
- Meharry Medical College