Biomarkers of Exposure to Toxic Substances: Volume 4: Metabonomics Biomarkers to Liver and Organ Damage

Abstract

In this study we examined the urinary metabolite profiles from rats following a single exposure to the kidney toxicants D-serine, puromycin, hippuric acid and amphotericin B at various doses, and as a function of time post-dose. In toxicology, such dose-time metabonomics studies are important for an accurate determination of the severity of biological effects, and for biomarker identification that may be associated with toxicity. The metabonomics analysis yielded a dose-response curve in principal component analysis space, and was able to detect exposure to D-serine and puromycin at much lower doses than standard clinical chemistry measures. Additionally, characteristic features in the urinary metabolite profiles could be ascertained as a function of dose. The results showed common features and some unique features in urinary metabolite profiles when analyzed by NMR and LC-MS, respectively.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2009
Accession Number
ADA530977

Entities

People

  • Andrew Neuforth
  • Deirdre Mahle
  • Harry Luithardt
  • Michael Raymer
  • Nestor Tarragona
  • Nicholas Delraso
  • Nicholas Reo
  • Paul Anderson
  • Randel Reitcheck

Organizations

  • Wright State University

Tags

Communities of Interest

  • Biomedical
  • Engineered Resilient Systems

DTIC Thesaurus Topics

  • Amines
  • Amino Acids
  • Chemical Synthesis
  • Chemistry
  • Computational Science
  • Data Analysis
  • Data Science
  • Databases
  • Factor Analysis
  • Hippuric Acid
  • Identification
  • Information Science
  • Liquid Chromatography
  • Mass Spectrometry
  • Metabolomics
  • Statistical Analysis
  • Surveys

Readers

  • Immunology
  • Nanoscale Plasmonic Nanotechnology
  • Prostate Cancer Biology.

Technology Areas

  • Space