Green Synthesis of D-1,2,4 - Butantetroil from D-Glucose
Abstract
The current microbial synthesis of D-1,2,4-butanetriol is based on the use of D-xylose as the starting material. Although D-xylose is abundant in hemicellulose, streams of D-xylose sufficiently pure to support microbial growth are not available in the U.S. Because of the current expense of D-xylose, a microbial synthesis of D-1,2,4-butanetriol from D-glucose was targeted for development. The activity of mdlC-encoded benzoylformate decarboxylase is essential to improving the yields and concentrations of microbe-synthesized D-1,2,4-butanetriol. Codon optimization of Pseudomonas putida mdlC for heterologous expression in an Escherichia coli host was therefore examined.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2008
- Accession Number
- ADA593490
Entities
People
- John W. Frost