Investigations Concerning Hydrolysis and Stabilization of Antiradiation Compounds

Abstract

This report contains results of the following studies: Encapsulation of ethiofos (WR 2721) for use as oral dosage forms. In vitro evaluation of the most promising samples. Development of analytical methodology for the active species. Preliminary pharmacokinetic results of in vivo studies. This report covers studies conducted on S-2-(3-aminopropylamino)ethyl phosphorothioic acid, ethiofos (WR 2721), over the period January 16, 1983 through January 13, 1984. The most significant results obtained during this report period are following. Plasma HPLC assay methods have been developed for ethiofos over the range of 0. 05 micrograms/mL to >1000 micrograms/mL. The improved ethiofos assay method has been used successfully in two dosing experiments with beagle dogs. An HPLC plasma assay method has been developed for WR 1065 over the range of 1 microgram/mL to >500 micrograms/mL. WR 1065 disappears quickly in dog plasma even at -78 C. Methods to stabilize the plasma samples have been investigated and some appear very promising. The WR 1065 assay method has been used in a preliminary ethiofos dosing study with a beagle dog.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1984
Accession Number
ADB094846

Entities

People

  • C. W. Lew
  • D. J. Mangold
  • E. P. Mcgovern
  • N. F. Swynnerton
  • T. M. Ludden

Organizations

  • Southwest Research Institute

Tags

DTIC Thesaurus Topics

  • Accuracy
  • Acids
  • Alcohols
  • Buffers (Chemistry)
  • Chemical Synthesis
  • Chemistry
  • Chromatographs
  • Chromatography
  • Detectors
  • Hydroxides
  • Jugular Vein
  • Liquid Chromatography
  • Materials
  • Measurement
  • Medical Personnel
  • Microcapsules
  • Veins

Fields of Study

  • Chemistry
  • Medicine

Readers

  • Analytical Chemistry
  • Toxicology/Environmental Toxicology